ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band observed in mouse kidney and liver lysates (calculated MW of 27.7 kDa according to mouse NP_001077398.1 [isoform1], 25.4 kDa according to mouse NP_032187.2 [isoform 2] and 25.5 kDa according to human NP_002075.2). Recommended concentration: 1-3 μg/mL.
GPX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GPX3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.