ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 38-40 kDa band observed in human cerebellum, liver and skeletal muscle lysates (calculated MW of 46.2 kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87 (9) :3484-8; PMID: 2185471). Recommended concentration: 0.01-1 μg/mL. Immunohistochemistry: In paraffin embedded human heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration, 2-3 μg/mL.
GOT1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GOT1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.