ELISA: Antibody detection limit dilution 1:16,000. In transfected HEK293 transiently expressing full-length human GOLM1 (myc and DYKDDDDK tagged), a band of approximately. 75 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows luminal staining of secretory cells in the glands. Similar results were obtained in human uterus and small intestine. Recommended concentration, 3-6 μg/mL.
GOLPH2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GOLpH 2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.