ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 170 kDa band observed in human HeLa lysates (calculated MW of 167 kDa according to NP_005886.2). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at appox 25 kDa . This band was successfully blocked by incubation with the immunizing peptide.
GOLGA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GOLGA3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.