ELISA: Antibody detection limit dilution >1/32,000. Western Blot: Approximately 55 kDa band in K562 lysate at 0.5 μg/mL. This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands. Recommended concentration, 3-6 μg/mL.
GCIP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GCIP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.