ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 40 kDa band observed in lysates of liver cell line HepG2 (calculated MW of 41.3 kDa according to NP_536721.1). Recommended concentration: 0.3-1 μg/mL.
GATA5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GATA5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.