ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 24-25 kDa and 37 kDa bands observed in lysates of rat spinal cord (calculated MW of 24.9 kDa according to rat NP_037090.1 and calculated MW of 38.9 kDa according to AAC52438.1 and sp Q62805). Recommended concentration: 0.1-0.3 μg/mL.
GALR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GALR1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.