ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 75 kDa band observed in HEPG2 lysate (calculated MW of 73.4 kDa according to NP_055145.2). Recommended concentration: 0.1-0.3 μg/mL.
GADD34 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GADD34 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse