ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 65+70 kDa bands observed in human brain lysates. an additional band of 26 kDa is observed. All bands were blocked by the immunizing peptide (calculated MW of 65.4 kDa according to NP_000809.1). Recommended concentration: 0.1-0.3 μg/mL.
GAD65 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GAD65 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.