ELISA: Antibody detection limit dilution 1:64,000. Approximately 48 kDa band observed in human kidney, liver and lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approximately 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.
Fumarase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Fumarase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.