ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 60 kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6 kDa according to NP_003498.1). Recommended concentration: 0.3-1 μg/mL.
Frizzled 7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Frizzled 7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.