ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Band at approximately 75 kDa in lysates of FOXP4-transfected HEK293 (calculated MW of 73.5 kDa according to NP_001012426.1). Recommended concentration: 1-3 μg/mL.
FOXP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FOXP4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.