ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 90-100 kDa band in lysates of human heart, muscle, colon, duodenum and pancreas after 1 μg/mL antibody staining.5 kDa according to NP_005241.1. The 90-100 kDa band was successfully blocked by incubation with the immunizing peptide.
FOXL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FOXL1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.