ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 60 kDa band observed in human liver lysates (calculated MW of 48.3 kDa according to NP_068556.1and NP_710141.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277 (20) :17564-70.; PMID: 11875061). Recommended concentration: 1-3 μg/mL.
FOXA2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FOXA2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.