ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in lysate of human epidermoid carcinoma A431 (calculated MW of 59.9 kDa (isoform A) according to NP_510965.1 and 58.2 kDa (isoform B) according to NP_055096.2). Recommended concentration: 1-3 μg/mL.
FIR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FIR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.