ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 30 kDa band observed in human muscle lysates (calculated MW of 31.9 kDa according to NP_001440). Recommended concentration: 0.05-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows fibrous staining in the cytoplasm of developing sperm cells. Recommended concentration, 2-2 μg/mL.
FHL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FHL1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse