ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 48 kDa band observed in human kidney lysates (calculated MW of 48.0 kDa according to NP_036296.1). Recommended concentration: 0.3-1.5 μg/mL. A stronger band of unknown identity was also consistently observed at appr. 30 kDa - both bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3 μg/mL.
FBXW2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FBXW2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.