ELISA: Antibody detection limit dilution 1:64000. Western Blot: A very strong Approximately 16 kDa band observed in human duodenum lysates (calculated MW of 15.2 kDa according to NP_000125.1). In transfected HEK293 transiently expressing FABP2 a band of approximately 18 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.001 μg/mL. Immunohistochemistry: In paraffin embedded human small intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration, 2-3 μg/mL.
FABP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FABP2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.