ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 50 kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1 μg/mL antibody staining.5 kDa, 67.1 kDa and 69.4 kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
EYA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EYA4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse