ELISA: Antibody detection limit dilution 1:16000. Western Blot: Consistent band observed in 293 and human heart at approximately 75 kDa with 1 hour incubation of primary at 0.5 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows staining of the syncytiotrophoblasts. Recommended concentration, 3-6 μg/mL.
EVC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EVC2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.