ELISA: Antibody detection limit dilution 1:4,000. Approximately 60 kDa band observed in human lung and placenta lysates (calculated MW of 110 kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem. 2007 Apr 10; PMID: 17428795). Recommended concentration: 1-3 μg/mL.
EPHB2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EpH B2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.