ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 70 kDa band observed in human tonsil, lymph node and in Jurkat T-cell line lysates (calculated MW of 67.5 kDa according to NP_758961.1). Recommended concentration: 0.3-1.0 μg/mL.
ELF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ELF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.