ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in human heart lysates (calculated MW of 61.2 kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approximately 62 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.003-0.01 μg/mL.
EHD2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EHD2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.