ELISA: Antibody detection limit dilution >1:32000. Western Blot: Approximately 35-40 kDa band observed in human testis, human muscle, A549 and A431 lysates at 0.5-1 μgof primary, this band was successfully blocked by incubation with the immunizing peptide. Please note that the band we observe is different from the predicted size of approximately 322 kDa according to NP_056986. Immunohistochemistry: In paraffin embedded human testis shows nuclear staining in . Recommended concentration, 1-2 μg/mL.
EDD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EDD1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.