ELISA: Antibody detection limit dilution >1:32,000. Western Blot: 100 kDa band reported in human U87-MG glioblastoma lysate at 2.5 μg/mL. A 30 kDa band of unknown identity was also observed.
ECT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ECT2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.