ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 26 kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4 kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3 μg/mL. A minor band of unknown identity was also consistently observed at 75 kDa . This band was successfully blocked by incubation with the immunizing peptide.
EBAG9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
EBAG9 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.