ELISA: Antibody detection limit dilution 1:16000. Western Blot: lysates of COS1 transfected with full length recombinant human DYX1C1 gave bands at approximately 48 kDa after 0.1 μg/mL antibody staining. In addition, a minor band of 24 kDa is detected consistent with observations with N-terminal specific antibodies. The 75 kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this antibody is used in other assays than Western Blot.
DYX1C1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DYX1C1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.