ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band in human brain (amygdala and cerebellum), kidney and liver lysates after 0.3 μg/mL antibody staining.. The 26 kDa band was successfully blocked by incubation with the immunizing peptide.
Dynamin 3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dynamin 3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.