ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human lysates of HeLa (calculated MW of 39.3 kDa according to NP_004408.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration, 3-5 μg/mL.
DUSP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DUSP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.