ELISA: Antibody detection limit dilution 1:32,000. Immunohistochemistry: In paraffin embedded human thyroid shows staining of cytoplasm in epithelial cells of the gland. Recommended concentration, 4-6 μg/mL.
Dual oxidase 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dual oxidase 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.