ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 50 kDa band observed in human kidney lysates and 48 kDa on human brain lysates (calculated MW of 53.9 kDa according to NP_000781.1). Recommended concentration: 0.03-0.1 μg/mL.
DOPA decarboxylase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DOPA decarboxylase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.