ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 70 kDa band observed in HEK293 lysate (calculated MW of 38.4 kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1;238(2):301-13.; PMID: 10570958 ). Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human brain cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration, 5-10 μg/mL.
DKK3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DKK3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.