ELISA: Antibody detection limit dilution 1:128,000. Western Blot: In transfected HEK293 transiently expressing DGAT2 bands of approximately 60 kDa and 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in human liver lysates. The calculated molecular size is 43.8 kDa according to NP_115953.2. Recommended concentration, 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3 μg/mL.
DGAT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DGAT2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.