ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 70 kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1 kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/mL.
DDX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DDX5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.