ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 75 kDa band observed in A431 and HeLa lysates (calculated MW of 63.3 kDa according to NP_060873.3). Recommended concentration: 1-3 μg/mL. an additional band of 48 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
DCP1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DCP1A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.