ELISA: Antibody detection limit dilution 1:4,000. Western Blot: full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approximately 52 kDa band observed (calculated MW of 52.8 kDa according to NP_057440.1). Recommmended concentration 0.1-0.3 μg/mL.
DCDC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DCDC2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.