ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 50 kDa band observed in lysates of human ovary and of cell line K562 (calculated MW of 50.5 kDa according to NP_001014451.1). Recommended concentration: 0.5-1.5 μg/mL.
DBP5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DBP5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.