ELISA: Antibody detection limit dilution 1:128,000. Approximately 40 kDa band observed in human spleen lysates (calculated MW of 9.5 kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30;285 (5428) :730-2.; PMID: 10426994). Recommended concentration: 0.2-0.6 μg/mL. an additional band of unknown identity was also consistently observed at 100 kDa . This band was successfully blocked by incubation with the immunizing peptide.
DAP10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DAP10 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.