ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 26 kDa band observed in human breast tumor lysates (calculated MW of 22.5 kDa according to NP_003278.1). Recommended concentration: 0.1-0.3 μg/mL. See ProSci's D52 and D54 products.
D53 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
D53 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.