ELISA: Antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments in human liver, kidney and prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL).Immunohistochemistry: In paraffin embedded human breast shows textured cytoplasm staining in ductal epithelial cells. Recommended concentration, 3-6 μg/mL.
CYP7B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CYP7B1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.