ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 50-55 kDa band in lysates of human thymus, kidney and placenta, and of cell line HeLa after 0.3 μg/mL antibody staining. The 50-55 kDa band was successfully blocked by incubation with the immunizing peptide.
CXCR3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CXCR3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.