ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 55 kDa band in human breast, placenta and testis lysates after 0.1 μg/mL antibody staining.6 kDa according to NP_115934.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
Cripto-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cripto-1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.