ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 65-70 kDa band observed in human brain lysates (predicted MW of 65 kDa according to NP_085097). Recommended for use at 0.5-2 μg/mL. Please note this band was always accompanied by a slightly stonger band at approximately 48 kDa and further both bands could be blocked by incubation with the immunizing peptide.
CPEB1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CPEB1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.