ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 60 kDa band observed in MOLT4 and Jurkat lysates (calculated MW of 51.0 kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 μg/mL.
Coronin 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Coronin 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.