ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 150 kDa band observed in rat spinal cord lysates (calculated MW of 133 kDa according to human NP_003604.2 and of 132 kDa according to rat NP_001101631.1). Recommended concentration: 0.1-0.3 μg/mL.
CILP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CILP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.