ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunizing peptide.
CHFR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CHFR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.