ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 30 kDa and 40 kDa bands in human pancreas lysates at 1 μg/mL.3 and 196 kDa according to NP_003598.2. Immunohistochemistry: In paraffin embedded human cerebellum shows cytoplasm staining in Purkinje cells. Recommended concentration, 3-6 μg/mL.
Cdc42-binding kinase alpha antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cdc42-binding kinase alpH a antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.