ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 34 kDa band observed in human myeloid erythroblast cell line K562 and human monocytic cell line U937 lysates (calculated MW of 34.0 kDa according to NP_003992.3;). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in human placenta. Recommended concentration, 3-6 μg/mL.
CD32 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CD32 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.