ELISA: Antibody detection limit dilution 1:32,000. Approximately 50 kDa band observed in human lymph node, thymus and tonsil lysates (calculated MW of 37.6 kDa according to NP_006101.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A. 1998 Dec 8;95 (25) :14897-902). Recommended concentration: 0.3-1.0 μg/mL.
CD2BP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CD2BP2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.