ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 26+28 kDa (doublet) and 40 kDa in human bone marrow and spleen lysates after 0.1 μg/mL antibody staining.The 26+28 kDa doublet was also found in human lymph node and in tonsil lysates.2 kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands).
CCL3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CCL3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.